bone marrow neutrophil isolation kits p8550  (Beijing Solarbio Science)

Journal: ACS Biomaterials Science & Engineering

Article Title: Construction of a 3D Degradable PLLA/β-TCP/CS Scaffold for Establishing an Induced Membrane Inspired by the Modified Single-Stage Masquelet Technique

doi: 10.1021/acsbiomaterials.4c01849

Figure Lengend Snippet: Analysis of cell viability on various bone scaffolds in vitro. (A) SEM pictures of 3D-printing scaffolds in 50× and 100× magnifications and cell adhesion (the white arrows indicate the BMSCs, the blue arrow designates the β-TCP nanoparticle and the orange arrow indicates the CS particle). n = 3/group, * P < 0.05, ** P < 0.01. (B) Fluorescence micrographs of live (green) and dead (red) cells which cultured 3 days on different scaffolds.

Article Snippet: Rabbit-derived bone marrow stem cells (BMSCs) were obtained from Wuhan Procell Life Technology Co., Ltd. (Wuhan, China).

Techniques: In Vitro, Fluorescence, Cell Culture

Journal: ACS Biomaterials Science & Engineering

Article Title: Construction of a 3D Degradable PLLA/β-TCP/CS Scaffold for Establishing an Induced Membrane Inspired by the Modified Single-Stage Masquelet Technique

doi: 10.1021/acsbiomaterials.4c01849

Figure Lengend Snippet: Cytocompatibility of various bone scaffolds. (A) Inverted fluorescence microscope images of BMSCs cultured with different scaffold extract fluids (40× magnification). (B) Optical density (OD) values of BMSCs cultured with various scaffold extract fluids along with statistical analysis. n = 5/group, ns means the difference is not statistically significant.

Article Snippet: Rabbit-derived bone marrow stem cells (BMSCs) were obtained from Wuhan Procell Life Technology Co., Ltd. (Wuhan, China).

Techniques: Fluorescence, Microscopy, Cell Culture

Journal: ACS Biomaterials Science & Engineering

Article Title: Construction of a 3D Degradable PLLA/β-TCP/CS Scaffold for Establishing an Induced Membrane Inspired by the Modified Single-Stage Masquelet Technique

doi: 10.1021/acsbiomaterials.4c01849

Figure Lengend Snippet: Osteogenic potential of IMs mediated by various filling materials. (A) ALP staining of BMSCs treated by proteins extracted from different IMs (magnification, ×100). (B) ARS staining of MSCs treated by proteins extracted from different IMs (magnification, ×100). n = 3/group, * P < 0.05, **** P < 0.0001.

Article Snippet: Rabbit-derived bone marrow stem cells (BMSCs) were obtained from Wuhan Procell Life Technology Co., Ltd. (Wuhan, China).

Techniques: Staining

Journal: Regenerative Therapy

Article Title: Developing engineered muscle tissues utilizing standard cell culture plates and mesenchymal stem cell-conditioned medium

doi: 10.1016/j.reth.2024.08.011

Figure Lengend Snippet: Paracrine factors from MSCs in conditioned medium influence myoblast function. Conditioned medium from MSCs enhance C2C12 proliferation (a), survival (b) (n = 4; means ± SD; ∗p < 0.05). (c) The decreased concentration of glucose in medium following 24 h culture of 12-day-old tissues; (n = 3; means ± SD; ∗p < 0.05). (d) Quantified mRNA expression of the myogenic differentiation related genes on Day 10 (n = 3; mean ± S.D, ∗p < 0.05, ∗∗∗p < 0.001, ns = not significant).

Article Snippet: The rabbit bone marrow mesenchymal stem cells (BM-MSCs) were obtained from Cyagen Biosciences and the growth medium was DMEM basic with 10% FBS and 1% P/S.

Techniques: Concentration Assay, Expressing

Journal: Polymers

Article Title: Magnesium Hydroxide as a Versatile Nanofiller for 3D-Printed PLA Bone Scaffolds

doi: 10.3390/polym16020198

Figure Lengend Snippet: ALP staining images of BMSCs after culturing for 7 and 14 days: PLA ( a , d ), PLA/5Mg(OH) 2 ( b , e ), PLA/20Mg(OH) 2 ( c , f ). Notes: More cells with a higher degree of staining are observed in PLA/5Mg(OH) 2 , but only a large amount of whisker-like substances are seen in PLA/20Mg(OH) 2 , indicating that the appropriate addition of Mg(OH) 2 is beneficial for enhancing the osteogenic differentiation ability of cells.

Article Snippet: The materials used in the experiments are mainly included: PLA (REVODE, Zhejiang Hisun Biomaterials Co., Ltd., Taizhou, China); Mg(OH) 2 nanopowders (~200 nm, as shown in ) (Beijing Deke Science and Technology Co., Ltd., Beijing, China); dichloromethane (CH 2 Cl 2 , ≥99.5%, Sichuan Xilong Science Co., Ltd., Chengdu, China); simulated body fluid (SBF) (R24167, 1.5×, Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China); rabbit bone marrow mesenchymal stem cells (BMSCs) (RBXMX-01001) and BMSC complete culture medium (RBXMX-80011) (Cyagen Biosciences, Inc., Guangzhou, China); DAPI staining solution (C1005), actin-tracker red-rhodamine (C2207S), BCIP/NBT alkaline phosphatase color development kit (C3206) (Shanghai Beyotime Biotechnology Co., Ltd., Shanghai, China); and phosphate buffered solution (Yida Technology Co., Ltd., Quanzhou, China).

Techniques: Staining, Whisker Assay